Plant & Animal Metabolism

Our metabolism group is furnished with all the latest in accurate mass platforms such as AB SCIEX QTOF X500B, Agilent QTOF G6530 and several other QTOF platforms.  Our mass spectrometers are interfaced to Model 5C Lab Logic radio detectors and Agilent’s latest UHPLC-1290 INFINITY II liquid chromatography systems. Our team utilizes Harvey Biological Material Oxidizers along with Beckman 6500 Series Liquid Scintillation Counters and Perkin Elmer MicroBeta-II with 12 detectors to screen samples for radioactivity traces.  For fraction collection (FC), we utilize both the traditional FC from Pharmacia as well as Agilent’s UHPLC-1290 INFINITY II Fraction collection into various well plates i.e., 96 well plates, HPLC vials etc.

In addition to many decades in-vitro and in-vivo metabolism experience on behalf of our metabolism chemistry staff, as well as all the latest technology in structure elucidation, Symbiotic Research, LLC fully owns its own in-life facility (the Genesis Midwest, LLC), this provides flexibility to our sponsors to choose study director from either site: Wisconsin or New Jersey. Reminder that FDA requires sponsors to select study directors only from the in-life location unless the in-life facility is fully owned. Under this circumstance, the in-life facility can operate out of a different location. This is a fringe benefit that our sponsor gets when it comes to choosing a particular study director which they are comfortable to work with from either location.

In a nutshell, we offer a turn-key comprehensive Metabolism study package to our sponsors with a variety of animal and plant species all under one ‘roof’: From in-life phase to quantitative & qualitative analyses:

From in-life phase to quantitative & qualitative analyses:

• 14C-licensed & AAALAC-accredited animal facility for in-life phase
• GLP- & Non-GLP-conform studies following international guidelines (US-EPA OPPTS/OCSPP, OECD, JMAFF, etc.)
• 14C-tracer and non-labeled studies
• Assessment of metabolic pathways in larger animals i.e. ruminant animals, poultry, and a wide variety of plants
• Confined & field rotational crops
• Preparation of analytical samples: Homogenization and choice of facility for determination of total radioactive residue (TRR) either at Genesis Midwest site or at Symbiotic Research site
• State-of-the-art identification of degradation products & metabolites via variety of mass spectroscopic instruments such as the latest in QTOF from AB SCIEX interfaced to HPLC or UHPLC & Capillary Electrophoresis (CE and CZE-MS-TOF)
• Capacity to set-up large bioaccumulation and fish metabolism studies
• Access to modern greenhouses through partnering companies and/or other internal global tentamus labs”

Bioconcentration & Metabolism – Characterization of potential of chemical substances to bioconcentrate in aquatic species.

We offer identification of metabolic pathway of substances in fish:

• GLP- & Non-GLP-conform studies following international guidelines (US-EPA OPPTS/OCSPP, OECD, SETAC, REACH etc.)
• 14C-tracer or non-radiolabeled studies, both at our analytical lab and our large in-life facility
• Evaluation of the potential uptake of test substance during exposure phase
• Assessment of the elimination of accumulated substances during post-exposure/depuration phase
• Identification of steady-state or kinetic bioconcentration factor for test substances and major metabolites
• Bioaccumulation studies with fish, crustacean, etc.
• Fish metabolism and fish feeding studies with cold and warm water species
• Total radioactive residue (TRR) determination in variety of fish tissues
• Fish lipid content determination
• In-life facility- Genesis Midwest, LLC to accommodate large bioaccumulation and fish metabolism studies utilizing static and semi-static designs