Instrumentation in Metabolism Chemistry

Our metab­o­lism group is furnished with all the latest in accu­rate mass plat­forms such as AB SCIEX QTOF X500B, Agilent QTOF G6530 and several other QTOF plat­forms. Our mass spec­trom­e­ters are inter­faced to Model 5C Lab Logic radio detec­tors and Agilent’s latest UHPLC-1290 INFINITY II liquid chro­matog­raphy systems. Our team utilizes Harvey Biolog­ical Mate­rial Oxidizers along with Beckman 6500 Series Liquid Scin­til­la­tion Coun­ters and Perkin Elmer Micro­Beta-II with 12 detec­tors to screen samples for radioac­tivity traces. For frac­tion collec­tion (FC), we utilize both the tradi­tional FC from Phar­macia as well as Agilent’s UHPLC-1290 INFINITY II Frac­tion collec­tion into various well plates i.e., 96 well plates, HPLC vials etc.

Why utilize Symbiotic Research Metabolism Services

In addi­tion to many decades in-vitro and in-vivo metab­o­lism expe­ri­ence on behalf of our metab­o­lism chem­istry staff, as well as all the latest tech­nology in struc­ture eluci­da­tion, Symbi­otic Research, LLC fully owns its own in-life facility (the Genesis Midwest, LLC), this provides flex­i­bility to our spon­sors to choose study director from either site: Wisconsin or New Jersey. Reminder that FDA requires spon­sors to select study direc­tors only from the in-life loca­tion unless the in-life facility is fully owned. Under this circum­stance, the in-life facility can operate out of a different loca­tion. This is a fringe benefit that our sponsor gets when it comes to choosing a partic­ular study director which they are comfort­able to work with from either location.

In a nutshell, we offer a turn-key compre­hen­sive Metab­o­lism study package to our spon­sors with a variety of animal and plant species all under one roof’: From in-life phase to quan­ti­ta­tive & qual­i­ta­tive analyses:

From in-life phase to quan­ti­ta­tive & qual­i­ta­tive analyses:

  • 14C-licensed & AAALAC-accred­ited animal facility for in-life phase
  • GLP- & Non-GLP-conform studies following inter­na­tional guide­lines (US-EPA OPPTS/OCSPP, OECD, JMAFF, etc.)
  • 14C-tracer and non-labeled studies
  • Assess­ment of meta­bolic path­ways in larger animals i.e. rumi­nant animals, poultry, and a wide variety of plants
  • Confined & field rota­tional crops
  • Prepa­ra­tion of analyt­ical samples: Homog­e­niza­tion and choice of facility for deter­mi­na­tion of total radioac­tive residue (TRR) either at Genesis Midwest site or at Symbi­otic Research site
  • State-of-the-art iden­ti­fi­ca­tion of degra­da­tion prod­ucts & metabo­lites via variety of mass spec­tro­scopic instru­ments such as the latest in QTOF from AB SCIEX inter­faced to HPLC or UHPLC & Capil­lary Elec­trophoresis (CE and CZE-MS-TOF)
  • Capacity to set-up large bioac­cu­mu­la­tion and fish metab­o­lism studies
  • Access to modern green­houses through part­nering compa­nies and/​or other internal global tentamus labs”

Underwater photography of The Red Tail Catfish - Phractocephalus hemiliopterus. This tropical fish is native to the Amazon, Orinoco, and Essequibo river basins of South America.

Bioaccumulation /
Fish Metabolism

Biocon­cen­tra­tion & Metab­o­lism – Char­ac­ter­i­za­tion of poten­tial of chem­ical substances to biocon­cen­trate in aquatic species.

We offer iden­ti­fi­ca­tion of meta­bolic pathway of substances in fish:

  • GLP- & Non-GLP-conform studies following inter­na­tional guide­lines (US-EPA OPPTS/OCSPP, OECD, SETAC, REACH etc.)
  • 14C-tracer or non-radi­o­la­beled studies, both at our analyt­ical lab and our large in-life facility
  • Eval­u­a­tion of the poten­tial uptake of test substance during expo­sure phase
  • Assess­ment of the elim­i­na­tion of accu­mu­lated substances during post-expo­sure/depu­ra­tion phase
  • Iden­ti­fi­ca­tion of steady-state or kinetic biocon­cen­tra­tion factor for test substances and major metabolites
  • Bioac­cu­mu­la­tion studies with fish, crus­tacean, etc.
  • Fish metab­o­lism and fish feeding studies with cold and warm water species
  • Total radioac­tive residue (TRR) deter­mi­na­tion in variety of fish tissues
  • Fish lipid content determination
  • In-life facility- Genesis Midwest, LLC to accom­mo­date large bioac­cu­mu­la­tion and fish metab­o­lism studies utilizing static and semi-static designs
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